To perform the assay of Metronidazole.

 Experiment/Assay

Object: 

To perform the assay of Metronidazole.

Structure:

IUPAC Name:

2-(2-methyl-5-nitro-1H-imidazol-1-yl) ethanol

Molecular Formula: 

C6H,N303

Mol. Wt.: 171.2.g/mol

Metronidazole contains not less than 99.0 per cent and not more than 101.0 per cent of C6H,N30, calculated on dried basis.

Category: Antiamoebic.

Dose: 

For trichomoniasis, 200 mg thrice daily, for 7 days. For amoebiasis, 400 mg thrice

daily, for 5 to 10 days. For giardiasis, 2 g daily for 3 successive days for adults, 1 g for

children and 400 mg daily for infants. Initial dose, by intravenous infusion 15 mg per kg of body weight; subsequent doses, 7.5 mg per kg of body weight upto a maximum of 1 g every six hours, for 7 days or longer.

Description: A white or yellowish, crystalline powder.

Assay:

•Weigh accurately about 0.15 g, dissolve in 50 ml of anhydrous glacial acetic acid.

•Titrate with 0.1 M perchloric acid, determining the end-point potentiometrically.

•Carry out a blank titration.

Factor: 1 ml of 0.1 M perchloric acid is equivalent to 0.01712 g of metronidazole.

Storage:

 Store protected from light and moisture.

Uses: 

It is used as antiamoebic drug.

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Reference:

Dr. Abhishek Tiwari, Dr. Rajeev Kumar " A practical book of medicinal chemistry" Nirali Prakashan Pune 2019 pp 69