Estimation of Hemoglobin by Sahali's method.

 Estimation of Hemoglobin by Sahali's method. HAP Practical (Human Anatomy and Physiology Practical)

IMG source: Google 

AIM: To determine the hemoglobin content in given blood sample.

MATERIALS: Hemometer, Single mark pipette, Distilled water, Needle, 

Spirit, Cotton, HCl.

THEORY: ... **Write it By self**

PROCEDURE: 

1.Take 1/10 HCl in the Hb tube upto the lowest mark ‘2’.

2. Prick the finger with needle and collect 20µl of blood sample with single 

mark pipette.

3. Place the Hb tube on working table for five minutes for the formation of 

hemin crystals.

4. Place the Hb tube in the compater/hemometer and add drop by drop of 

distilled water into it until the colour of the solution in the Hb tube coincides 

with the glass plates of the compater.

5. If the colour coincides with the glass plates of the compater, observe the 

reading in the Hb tube. The percentage of Hb can be calculated from the 

reading.

DATA ANALYSIS:

Hb content in grams X 100 / 14.5

NORMAL VALUES:

 Males = 14 -18 grams 

Females = 13 - 14 grams 

Children = 10 - 13 grams

RESULT: The hemoglobin content present in given blood sample is_______